Proses Produksi Asam Sitrat Melalui Fermentasi: Metode dan Strategi: Proses Produksi Asam Sitrat Melalui Fermentasi: Metode dan  Strategi

Nanda Aurelia Salsabila; Cicilia Putri Meylani

Authors

Nanda Aurelia Salsabila Universitas Singaperbangsa Karawang
Cicilia Putri Meylani

Keywords:

Citric acid, Fermentation, Substrates, Microbes, Applications

Abstract

Citric acid, a widely used organic acid, is predominantly produced through fermentation by microorganisms. This review article provides an overview of the citric acid production process, focusing on the methods and strategies employed in fermentation. It explores various fermentation strategies, including Liquid Surface Fermentation Cultures, Solid-State Fermentation Cultures, and Submerged Fermentation, and their optimization for citric acid production. The choice of fermentation strategy is influenced by factors such as desired yield, available resources, and environmental considerations. The article also emphasizes the importance of post-fermentation extraction and purification stages, particularly precipitation methods like calcium citrate precipitation, which effectively recover high-purity citric acid. Further purification steps, such as ion exchange and crystallization, are discussed as means to achieve the desired purity level. The review concludes by highlighting the diverse properties of citric acid, including its preservative, flavoring, and pH-regulating abilities, which make it essential in various consumer products. It also underscores the ongoing research efforts to optimize production and purification processes, ensuring a sustainable and efficient supply of citric acid for industrial applications.

References

Scheele, C. W. (1784). Anmärkning om Citron-Saft, samt sätt att crystallisera den samma. Note on lemon juice, as well as ways to crystallize the same). Kongliga Vetenskaps Academiens Nya Handlingar, 5, 105-109.

Nangare, S., Vispute, Y., Tade, R., Dugam, S., & Patil, P. (2021). Pharmaceutical applications of citric acid. Future Journal of Pharmaceutical Sciences, 7, 1-23.

A. Amato, A. Becci, and F. Beolchini, “Citric acid bioproduction: the technological innovation change,” Critical Reviews in Biotechnology, vol. 40, no. 2. Taylor and Francis Ltd, pp. 199–212, Feb. 17, 2020.

Al-Qahtani, K. M. A. (2017). Extraction heavy metals from contaminated, water using chelating agents. Oriental journal of Chemistry, 33(4), 1698.

Salihu, R., Abd Razak, S. I., Zawawi, N. A., Kadir, M. R. A., Ismail, N. I., Jusoh, N., ... & Nayan, N. H. M. (2021). Citric acid: A green cross-linker of biomaterials for biomedical applications. European Polymer Journal, 146, 110271.

Lambros, M., Tran, T., Fei, Q., & Nicolaou, M. (2022). Citric acid: A multifunctional pharmaceutical excipient. Pharmaceutics, 14(5), 972.

Goel, A., Zhao, Z., Sørensen, D., Zhou, J., & Zhang, F. (2016). Esterification of pseudoephedrine hydrochloride by citric acid in a solid dose pharmaceutical preparation. Journal of Pharmaceutical and Biomedical Analysis, 129, 410-418.

Ipci, K., Öktemer, T., Birdane, L., Altintoprak, N., Muluk, N. B., Passali, D., ... & Cingi, C. (2016). Effervescent tablets: a safe and practical delivery system for drug administration. ENT updates, 6(1), 46. DOI:10.2399/jmu.2016001009

Obot, I. B., Meroufel, A., Onyeachu, I. B., Alenazi, A., & Sorour, A. A. (2019). Corrosion inhibitors for acid cleaning of desalination heat exchangers: Progress, challenges and future perspectives. Journal of Molecular Liquids, 296, 111760. Obot, I. B., Meroufel, A., Onyeachu, I. B., Alenazi, A., & Sorour, A. A. (2019). Corrosion inhibitors for acid cleaning of desalination heat exchangers: Progress, challenges and future perspectives. Journal of Molecular Liquids, 296, 111760.

Behera, B. C., Mishra, R., & Mohapatra, S. (2021). Microbial citric acid: Production, properties, application, and future perspectives. Food Frontiers, 2(1), 62-76.

He, Z.; Umemura, K. Utilization of citric acid in wood bonding. In Bio-Based Wood Adhesives: Preparation, Characterization, and Testing; He, Z., Ed.; CRC Press: Boca Raton, FL, USA, 2017; pp. 221–238.

Apelblat, A. (Ed.) Citric acid chemistry. In Citric Acid; Springer: Cham, Switzerland, 2014; pp. 213–266.

Oliveira, M. L. N., Malagoni, R. A., & Franco Jr, M. R. (2013). Solubility of citric acid in water, ethanol, n-propanol and in mixtures of ethanol+ water. Fluid Phase Equilibria, 352, 110-113.

Behera, B. C. (2020). Citric acid from Aspergillus niger: a comprehensive overview. Critical Reviews in Microbiology, 46(6), 727-749.

L. Vandenberghe, C. Rodrigues, and A. Pandey, “New Perspectives for Citric Acid Production and Application.” [Online]. Available: https://www.researchgate.net/publication/228338285

Show, P. L., Oladele, K. O., Siew, Q. Y., Aziz Zakry, F. A., Lan, J. C. W., & Ling, T. C. (2015). Overview of citric acid production from Aspergillus niger. Frontiers in life science, 8(3), 271-283.

Tao, Z., Yuan, H., Liu, M., Liu, Q., Zhang, S., Liu, H., ... & Wang, T. (2023). Yeast extract: characteristics, production, applications and future perspectives. Journal of microbiology and biotechnology, 33(2), 151.

Jacob, F. F., Striegel, L., Rychlik, M., Hutzler, M., & Methner, F. J. (2019). Spent yeast from brewing processes: a biodiverse starting material for yeast extract production. Fermentation, 5(2), 51.

Grewal, H. S., & Kalra, K. L. (1995). Fungal production of citric acid. Biotechnology advances, 13(2), 209-234.

Li, C., Xu, D., Zhao, M., Sun, L., & Wang, Y. (2014). Production optimization, purification, and characterization of a novel acid protease from a fusant by Aspergillus oryzae and Aspergillus niger. European Food Research and Technology, 238, 905-917.

Chen, H., He, X., Geng, H., & Liu, H. (2014). Physiological characterization of ATP-citrate lyase in Aspergillus niger. Journal of Industrial Microbiology and Biotechnology, 41(4), 721-731.

Singh Dhillon, G., Kaur Brar, S., Verma, M., & Tyagi, R. D. (2011). Recent advances in citric acid bio-production and recovery. Food and Bioprocess Technology, 4, 505-529.

Darouneh, E., Alavi, A., Vosoughi, M., Arjmand, M., Seifkordi, A., & Rajabi, R. (2009). Citric acid production: Surface culture versus submerged culture. African Journal of Microbiology Research, 3(9), 541-545.

Costa, J. A., Treichel, H., Kumar, V., & Pandey, A. (2018). Advances in solid-state fermentation. In Current developments in biotechnology and bioengineering (pp. 1-17). Elsevier.

Drysdale, C. R., & McKay, A. M. (1995). Citric acid production by Aspergillus niger in surface culture on inulin. Letters in applied microbiology, 20(4), 252-254.

Lende, S. V., Karemore, H., & Umekar, M. J. (2021). Review on production of citric acid by fermentation technology. GSC Biological and Pharmaceutical Sciences, 17(3), 085-093.

Ge, X., Vasco-Correa, J., & Li, Y. (2017). Solid-state fermentation bioreactors and fundamentals. In Current developments in biotechnology and bioengineering (pp. 381-402). Elsevier.

Książek, E. (2023). Citric Acid: Properties, Microbial Production, and Applications in Industries. Molecules, 29(1), 22.

Gaikwad, V., Panghal, A., Jadhav, S., Sharma, P., Bagal, A., Jadhav, A., & Chhikara, N. (2018). Designing of Fermenter and its utilization in food industries.

Li, Q. Z., Jiang, X. L., Feng, X. J., Wang, J. M., Sun, C., Zhang, H. B., ... & Liu, H. Z. (2016). Recovery processes of organic acids from fermentation broths in the biomass-based industry. Journal of microbiology and biotechnology, 26(1), 1-8.

Cavallo, E., Charreau, H., Cerrutti, P., & Foresti, M. L. (2017). Yarrowia lipolytica: a model yeast for citric acid production. FEMS yeast research, 17(8), fox084.

Lee, S. C., & Kim, H. C. (2011). Batch and continuous separation of acetic acid from succinic acid in a feed solution with high concentrations of carboxylic acids by emulsion liquid membranes. Journal of membrane science, 367(1-2), 190-196.

Ali, S. R., Anwar, Z., Irshad, M., Mukhtar, S., & Warraich, N. T. (2016). Bio-synthesis of citric acid from single and co-culture-based fermentation technology using agro-wastes. Journal of Radiation Research and Applied Sciences, 9(1), 57-62.

Fiume, M. M., Heldreth, B. A., Bergfeld, W. F., Belsito, D. V., Hill, R. A., Klaassen, C. D., ... & Andersen, F. A. (2014). Safety assessment of citric acid, inorganic citrate salts, and alkyl citrate esters as used in cosmetics. International journal of toxicology, 33(2_suppl), 16S-46S.

Proses Produksi Asam Sitrat Melalui Fermentasi: Metode dan Strategi